Transformation + microscope work

6-21-10
Out for wedding

6-22-10
High School Intern lunch, lab meeting, SEM class

6-23-10
Re-made LBAmp plates with 2x as much Amp as per Rick. The recipe in the MEDIA folder indicates 2mL of 25ug/ml per 1L of media solution, however it should be 2mL of 50ug/ml per 1L of media solution. For all future plates, recipe has been corrected.
SEM class
Transformation procedure completed for all 1-4. Plates incubated overnight at 37C

6-24-10
Picked colonies onto "Millenium Falcon" plates.
SEM class
Discussed upcoming Yellowstone trip, as well as details of upcoming extractions with Wendy

6-25-10
PCR reaction of 10 colonies chosen from sample 4. Proof of completed transformation is the purpose of this. We will run the results on a gel and hope that there are bands around the 1478kb range. + and - controls used. Only difference from normal PCR is that instead of 5ul we used toothpick removal and addition to the samples. PCR run for normal 3 hours using the same parameters as before.
Brown Bag
Gel to confirm results