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Week 9
Submitted by Bill Odette on August 10, 2009 - 6:41pm
Wrapping up. One of our Bacillus strains carrying the Fnr-1-deletion narG promoter showed no aerobic lacZ activity (what we expected), while the other strain did show aerobic activity. Two more strains we tested showed the same results. Unfortunately I won't have time to do everything required to determine why this happened-I'll be performing another lac assay monday and tuesday to test the hypothesis that nucleotide spacing is responsible for anaerobic narG transcription, finishing my presentation, and that's about it.
