WEEK 8

This past week I extracted a sample Lydie collected on July 21st from Ilwaco Bay that showed slight red water coloration. I ran a few PCR reactions with several samples from Ilwaco, Willapa and La Push. Unfortunately, I did not have any amplification occur. On Thursday and Friday I purified all the samples using a gel purification technique (I basically just cut out the DNA band from the gel to isolate it). I am anxious to work with the gel purified samples to see if they produce better results with the PCR! 

This past week I also received a few sequences back from the primate center for the cryptophyte rubisco primers and the M. rubra primers using samples from the red water blooms in 2007 and 2008. The ITS sequences for the M. rubra turned out to be ciliate sequences but not specifically M. rubra. The cryptophyte sequences however produced more appropriate results so this weekend the reverse is being sequenced so we can align the two together to get the full picture.