Week Seven

 The Amoa primer reaction was somewhat successful since the bands indicate a definite presence of the Amoa gene in 2/3 of the cDNA samples. However, the locations of the bands on the gels indicate strands are present that are longer than expected. I had been about to investigate this a little more but the primers I designed arrived and I had been excited to use those in an experiment. The initial reaction run showed a slight amplification in only one of the six new primers which is very frustrating. On friday I set up a new reaction using extracted DNA samples rather than the cDNA and will run it on a gel monday.

I also had to run a reaction along a temperature gradient that ranged from 57 to 65 degrees C twice. Since the first try was unsuccessful. However, the second try showed distinct bands which was a good sign and most looked like they matched up with the expected sizes. There was also a meeting with Jon and Holly thursday in which I was told to investigate rubisco and nifH primers for future experiments.