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Primers Primers and more Primers
Submitted by Matthew Geniza on July 24, 2010 - 7:02pm
Since the primers that Pete and I have been using start from the ITS 2 region, the sequences that we receive only account for about the first 400 base pairs of the 28S sequence. Pete and I developed internal primers so we could possibly obtain the full 28S sequence. After using these primers in PCR we could see that we were not getting the right size fragment. Next week we will try and optimize the PCR program to give us the right product.
In other news, the pneumonia that was plaguing me at the beginning of summer seems to be gone! In a previous trip the CMOP interns went to Multnomah Falls and climbed to the top of the falls. At the time is was unable to do so. However this week I was able to make it all the top - wheezing free!
