You are here

Blogs

Week 3 - Getting Better with this CompuCell3D Thing

Monday, 06/23/14:
    I read more from the CompuCell3D manual and found something that may help me with the mitosis simulation.

Week 2- More of PDFs, CompuCell3D

Monday, 06/16/14:
    Read more from PDFs about and practiced creating two dimensional illustrations of mitosis from one to 45 cells, had a meeting with Karen regarding progress and discussing how CompuCell3D works. I found a tutorial for mitosis called steppableBasedMitosis in my CompuCell3D folder that may help.

Week 1 - A New Beginning

I'm here for another summer of CMOP research! I'm looking foward to learning and producing some useful research!

So Little Time, So Much to Do

The fourth week of the program was a short week where I only worked three days in the lab. During these three days we started the control experiment for the liquid manifold. However, the 24 hour experiment failed once again! It appears that the Labview program for the manifold needs altering to collect the data after 16 hours. This process shall take a while to look through the program thoroughly. So, instead of restarting the 24 hour experiment, I began my side project using the liquid manifold for the pH stat.

Week 4: More Manganese

This week I continued to study manganese ligands. After a lot of research, I had the opportunity to try a different way of making manganese oxalate than the procedure we used last week. The new method produced a dark pink solution of manganese oxalate, and it was stable for about 30 minutes.

Week 2 - Debugging, segfaults, and progress

​This week, I spent most of my time with the python debugger, going line by line through my code to see why everything wasn’t working as it should. After some consideration, I decided to approach the data processing part in a different way. Turns out, the processing library I had been looking through already contained utilities to extract station data. However, I hit a brick wall when I started getting cryptic segfault errors. I spend the rest of the day Monday trying to get this code to work, but to no avail.

Week 5: Lots of Sampling!

This week started off with a whirlwind of sampling! I spent all of Monday sampling from our Welch Island and Whites Island sampling sites. It was very exciting to see the sites we collect from to obtain the samples I have been working with. Claudia worked with researchers from NOAA to collect samples, and I processed them out in the field, which was both exciting a somewhat scary because I was processing the samples alone and didn't want to do anything wrong. Fortunately, all the processing went smoothly, and we were able to get all the necessary information from both our sites.

Week 4: A Watched Cuvette Never Turns Blue

Like last week, this week was a blur. Monday lab meeting was productive; right away I knew it’d be bustling week. It started with freezing mixed probe (indigo disulfonate) and iron oxide (lepidocrocite) solutions. Freezing the samples at different rates may impact their redox conditions upon thawing. I continued absorbance tests on Monday as well.

Weeks 4 & 5: Sampling and Bonneville Dam!

I've been so busy here in the lab that some time has passed since I last gave updates on my progress, but I will summarize what I've been up to in these last couple of weeks. The beginning of my fourth week in the lab was very busy- we were preparing all the supplies necessary to go sediment sampling very early in the morning on Wednesday! The Senior Research Assistant Lydie was out for the week, so the task of organizing and arranging everything fell to me, my mentor Kiley, and Lydie's intern Maddie.

Week 5: Finally Getting Results

This week I once again tried to get some decent qPCR results.  My first run did not go well at all, and I ended up with my worst efficiency to date.  When analyzing my data, it seemed that my pipetting was fairly accurate, but the cycle threshold values were consistently off, indicating a problem with the preparation of the standard solutions rather than error in pipetting technique.  However, I made new standards on Wednesday, and after great anticipation I was finally rewarded with an efficiency value of 99%.

Pages

Subscribe to RSS - blogs