Week 2

This week has been frustrating. My project revolves around a stretch of DNA, and determining how exactly that stretch of DNA regulates gene expression. Before we can determine that, however, we need to isolate and clone several fragments containing different lengths of the DNA in question (the B. anthracis fnr promoter region). I've been trying to isolate these fragments this week, but have run into several problems with the plasmid vectors. I'm currently trying to purify the fragments of interest and ligate them into a plasmid we've had more success with, so hopefully once that has been successfully completed I'll be able to move forward into the next stage of the project.