WEEK 4

This week I began using my new primer, however to my disappointment I haven't had any results. Today I tried out many different concentrations of the DNA template so we will see if this makes a difference!

This week I also got to go sampling! Although it was a long day filled with lots of driving along windy highways, we had a great time! The weather was beautiful and the views and area we were driving through were truly breathtaking.   We even saw several does and a fawn along the highway. Unfortunately, while looking at our samples through the flowcam we didn’t see any Myrionecta like we were hoping.  It was still a successful trip though since I learned how to work the filtering equipment, gather samples and begin the chemical analysis. I have attached the sampling report for the trip.
 

Other than working with my new primer and sampling I have been preparing 16s and 18s PCR triplicates of samples from the May 24, 2009 cruise in order to clone them next week.