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Week 8 (2009)

We finally completed bryostatin labeling on my B.pacifica samples! My mentor and I continually put it on our "to  do" list but another experiment always got in the way. Anyway, over last week, I finished bryostatin labeling on B.pacifica larvae and ovicells, and B.neritina larvae. I need to spend more time looking at negative controls before I can come to any conclusions about my positive samples... but let's just say it looks like we have some exciting results (look for them in my presentation / paper)!

I also finished cloning, a process which will ultimately determine the sequences of the B.pacifica symbiont (a bacterium that is closely related to E.sertula).

GOALS FOR NEXT WEEK:
- Another DNA extraction to verify that the bryozoan is B.pacifica
- Work my paper/presentation (only 2 weeks left)!
- Look at my sequences to determine the identity of the symbiont