Week 8

 The week started with me running the gel for the 24x PCR reaction that tested all of the available primers and the pooled DNA extract template. Unfortunately this was unsuccessful for almost all of my primer sets I ran several other experiments to make sure that nothing was wrong with my methods. Yet, while I was able to get amplification throughout the week I had still been unable to get the 16s primers to amplify so I will need to continue testing to see if I can get that to happen. I was also able to test the cDNA and extracted DNA samples for rubisco thanks to Rachel Warnick who gave me several primers to test. This proves that there is something in the cDNA samples that I had been unable to amplify anything from previously. To end the week I ran an experiment to test the 16s primers by using the extracted DNA template and DNA Jon got from working with the bioreactor with hopes determining whether the 16s primers work or not.