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Week Eight - RNA and Microarray

On Monday, Tuesday, and Wednesday I did RNA isolations. Thursday I read and learned about microarray analysis. On Friday, I installed the necessary software for microarray analysis and tried to analyze (for the second time) the 12K microarray scans on my own and it was a success!

The (Mariya's) procedure for analysis goes like this:
1. Using combimatrix imager, open your image (the scan of the chip) and the probe design.
2. Use a template to align a grid to spots and extract data and save to desktop.
3. Using Blist (or probe weaver) normalize the extracted data from all scans together. Then you can view a histogram prior to or after normalization to see how the distribution of intensity is normalized. Finally, save the results of the normalized data.
4. Using BRB array tools, import the data and transform it into log data for further analysis (like scatter plots).

Also, this past weekend Ethan took us to Elliot Glacier, which was an awesome trip! Thank you Ethan! I had a great time. Here is the picture of our group.