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Trying Out the Hydrogen Peroxide Assay - Week 2

This week, most of my time was spent on practicing with hydrogen peroxide – A-red assay, a couple of Leucoberbelin Blue (LBB) assay to build calibration curves I also worked with Kati Geszvain and Christine Romero. They let me work a little bit with bacteria, such as streaking plates, inoculating bacteria from plates to liquid media, sterilize media and pipette tips in the steam sterilizer and oven.  As I said last week there were some complications in this protocol procedure. My mentor, Matthew Jones, and I had been working on clarifying out the procedure. Finally, we got the procedure straighten out.

The first three days of this week I spent on running the hydrogen peroxide assay and building up a calibration curve from the data collected. The first time I tried to run the assay, it took me three hours just to prepare the solution needed. It was because I had to wait for the working reagent that was make in DMSO to melt. The next few times I took out the DMSO first and let it melt while I prepare the working solutions. The last time I did this assay, it only took me one hour.

On Friday, I finished building up my calibration curves and some back-calculation using the best fit equation. Then I read more papers. After that, there was an kickball event out on the south lawn of Cooley Science Center. There were staff, faculties, interns, and postdocs. Everyone were so into the game and were like a big family. We had so much fun.

javascript:void(0)This is a 96 well plate used for the H2O2 Assay.