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Week 8- single-stranded targets

This week I tried to make single-stranded targets by running double-stranded PCR and then using a digestive enzyme (endonuclease) to end up with a single-stranded product.  However, the initial PCR reaction did not work, possibly due to degraded template. 
The purpose for single-stranded targets is to get a comparision between ds and ss targets.  ds targets are economically more viable because they take less time to produce.  ss targets are the standard, and so if we can show that ds data matches or is close to ss data, then we have optimized DNA assays.

At SHARP I have completed by first goal of finding parameters to run the dies with and am now helping them with testing different functionalization techniques and surface cleaning. 

-Vena