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Week 6- More optimizing
Submitted by Vena Haynes on July 19, 2010 - 1:56pm
This last week was pretty basic. I continued to try new DNA and buffer concentrations on the biosensors, and found that 0.5µg of ds DNA target works the best. This is promising because it is the lowest concentration we tried, meaning that our samples will last longer. The data on the different buffer concentrations was inconsistent and therefore inconclusive. This week we will try different temperatures because the 52°C we have been using for double-stranded targets may affect the adhesive on the channels after multiple runs.
Overall, we got some pretty nice data for the ds DNA targets I prepared on sensors that had not been used previously.
-Vena
