Week 5- Optimizing impedance sensor arrays

This week I was at SHARP and helped in the optimization of the impedance sensors.  Due to inconsistent data, different measures are being taken to figure out the problem.  We first began running the sensors at different frequency settings, and are now changing the concentrations of buffer and DNA targets, as well as different temperature controls.  This optimization usually is done using short oligonucleotide strands, but some of the chips were run with ds DNA targets I created here at CMOP. 

Attached is a run using 2.5ug ds targets of ADK, Hly and Stx2 on an ADK-specific chip.  You can see that the channel injected with ADK has a higher impedance than the channels injected with Hly and Stx, and the next two slides show the difference between an injection of either Hly and ADK or Stx and ADK (and the response increases due to changes in specificity)
       

-Vena