Week 9

This week I finished up hybridizing the last 12 samples so now we have all the data we need for a paper.  Mariya is gone this week so I started my paper once done with the lab work. In addition, I also helped Michelle Maier from Tawnya Peterson's lab with PCR product labeling and hybridizing as she will be performing these steps on board in September when the team goes out on a research cruise. So for next week I will finish up the paper and collaborate with Mariya for data analysis.  Also my final presentation is Fri the 14th!

Below is an actual heat map made by Mariya (specifically for marine Crenarchaeota) using a set of software to combine all the desired microarray data from each sample. On the far right is a dendrogram which represents phylogenetic relationships between group species; the multicolored list of words corresponds to each sample; and the actual heat map is divided into probes (x-axis) to which each sample (y-axis) was hybridized or not.  This definitely takes a while to get used to!