Week 9 - Results of the MS/MS

Last week, we ended up getting back our results from both the MS/MS, and the genetic sequencing lab.

The MS/MS gave us fairly strange results which suggested that the column in our lab may have been contaminated with proteins from a strain of bacteria 9A1 (arantimonus). This seems unlikely since great care was taken to clean the column, but to ensure reliable results, more investigation will be necessary; more samples will be run through the FPLC and the results of the MS/MS of these samples will be compared to the original results. Hopefully it turns out that the bacteria we are studying have Mn-oxidizing protiens that are similar to arantimonus, as opposed to contaminated with 9A1.

The genetic sequencing information we received yielded a few good sequences. Some of the data we got was extremely noisy and it was almost impossible to make conclusions about the data because it was so unreliable. A few of the sequences came back with great results, so those will be analyzed and worked on this week.

Today I made some media for other growth experiments that will be done soon, but the rest of the week will be spent working on my presentation and finishing up my final paper! only two weeks left!